Sedimentation equilibrium
From Free net encyclopedia
Sedimentation equilibrium is an analytical ultracentrifugation method for measuring protein molecular masses in solution and for studying protein-protein interactions. Particular applications of this technique are:
- measuring the equilibrium constant for association of proteins which reversibly self-associate to form oligomers.
- measuring the stoichiometry of complexes between two or more different proteins (e.g. a soluble receptor and its ligand or an antigen-antibody pair), or between a protein and a non-protein ligand.
- measuring the equilibrium constants for reversible protein-protein and protein-ligand interactions (approximate range 1 nanomolar to 1 millimolar).
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